脂质体对JEV－E糖蛋白的佐剂作用研究

Immunoadjuvant action of liposomes for entrapped Japanese En-cephalitis virus E-glucoprotein

目的：观察脂质体对日本脑炎病毒Ｅ糖蛋白的佐剂作用；方法：利用改进的振荡法，使亲和层析提取的日本脑炎病毒Ｅ糖蛋白包裹于脂质体中，制备出脂质体－Ｅ蛋白结合物，并分组免疫ＢＡＬＢ／ｃ小鼠；通过ＥＬＩＳＡ，血凝抑制试验（ＨＩ）和中和试验（ＮＴ）等指标观察．结果：脂质－Ｅ蛋白免疫组的特异性抗体水平明显高于游离Ｅ蛋白组；在两批病毒攻击保护性试验中，前者保护率分别为５０％和５７％，后者分别为３８％和３０％．结论：脂质体对日本脑炎病毒Ｅ糖蛋白有明显的佐剂作用．

Objective:In order to evaluate the adjuvantity of liposomes. Methods:Japanese Encephalitis virus(JEV) E-glucoprotein purified by affinity chromatography was encapsulated in the liposomes by hand shak-ing.Three groups of BALB/c mice were immunized peritoneally with JEV-E glucoprotein entrapped in lipo-somes(LE ),free E(FE glucoprotein and“ empty”liposomes(EL)respectively.The specific antibody was detected by ELISA,hemagglutination inhibition (HI)and neutralization test(NT)dynamically and the pro-tection rate of virus attack was observed.Results:The results show that the specific antibody level and pro-tection rate of the LE group are higher than those of the FE,In two batches of virus attack, the protection rates of the free E-protein group are 38%and 30%and those of the entrapped E-protein group are 50%and 57%. Conclusion:The study suggests that liposomes can promote the antigenicity of JEV E-glucoprotein.