胃癌耐药细胞株耐药谱的体外实验

In vitro study of anti-cancer agents resistant patterns of resistant hu-man gastric cancer cell lines

目的：观察胃癌细胞与抗肿瘤药作用后对不同药物的耐受情况．方法：胃癌细胞系ＳＧＣ７９０１在体外分别与长春新碱（ＶＣＲ），５氟尿嘧啶（５－Ｆｕ），阿霉素（ＡＤＭ）及氨甲喋呤（ＭＴＸ）作用，获得ＳＧＣ７９０１／ＶＣＲ，ＳＧＣ７９０１／ＡＤＭ，ＳＧＣ７９０１／５－Ｆｕ及ＳＧＣ７９０１／ＭＴＸ４个耐药细胞株．体外细胞毒性实验观察它们对ＶＣＲ，ＡＤＭ，５－Ｆｕ，ＭＴＸ，顺铂（ＣＤＤＰ）以及丝裂霉素以Ｃ（ＭＭＣ）的敏感性．结果：４株细胞对ＶＣＲ，ＭＴＸ，５－Ｆｕ及ＭＭＣ均有耐药，耐药指数为２．９～１５８３．ＳＧＣ７９０１／ＶＣＲ和ＳＧＣ７９０１／ＡＤＭ对ＡＤＭ分别呈１．６倍和１０１倍耐药．ＳＧＣ７９０１／ＡＤＭ和ＳＧＣ７９０１／５－Ｆｕ对ＣＤＤＰ呈６．３和２．５倍耐药．结论：４株胃癌细胞均产生了多药抗药性（Ｍｕｌｔｉｄｒｕｇｒｅｓｉｓｔａｎｃｅ，ＭＤＲ），药物引起的耐受可分为单向性交叉耐药及双向性交叉耐药两种类型．

bjective:The resistant patterns of acquied resistant of gastric cancer cell lines to anticancer drugs were investigated.Methods:Four resistant sublines,SGC7901/VCR,SGC7901/5-Fu,SGC7901/ADM,SGC7901/MTX were established with gastric cancer SGC790l cell separately selected by vincristine(VCR),5-fluorouracil(5-Fu),adriamycin(ADM)andmethotrexate(MTX)in vitro.The susceptibility of sublines to VCR,ADM,5-Fu,MTX,cisplatin(CDDP)and mitomycin(MMC)were studied using MTT methods.Re-sults:Four resistant sublines were cross resistant to VCR , MTX , 5-Fu and MMC, resistant index varying from 2.9 to 1583.SGC7901/VCR and SGC7901/ADM cells were resistant to ADM by 1.5 and 101-fold re-spectively while SGC7901 /ADM and SGC7901/5-Fu resistant to CDDP by 6.3 and 2.5-fold.Conclusion:Four sublines are all multidrug resistance(MDR).Anticancer drugs can cause one-way cross resistance and two-waycross resistance.