恶性肿瘤患者和正常人外周血单个核细胞培养上清SIL－2R水平的动态变化

Dynamic comparison of supernatants of peripheral blood mononucle-ar cell culture between malignant tumor patients and normal donors

作者测定２０例恶性肿瘤患者和２０例正常人血清中ＳＩＬ－２Ｒ水平，并对其外周血单个核细胞（ＰＢＭＣ）不加激活剂培养，加ＰＨＡ培养及加ｒＩＬ－２培养后３种情况上清中ＳＩＬ－２Ｒ水平的动态变化进行测定．结果表明，恶性肿瘤患者血清中ＳＩＬ－２Ｒ水平比正常人显著升高（Ｐ＜０．０１）；恶性肿瘤患者和正常人的ＰＢＭＣ不加激活剂培养的上清中ＳＩＬ－２Ｒ水平均略有增加，且正常人高于恶性肿瘤患者．加ＰＨＡ或者加ｒＩＬ－２激活培养的上清中ＳＩＬ－２Ｒ水平上升很高，ＰＨＡ激活培养的ＰＢＭＣ上清中ＳＩＬ－２Ｒ水平要比ｒＩＬ－２激活的ＰＢＭＣ培养上清中ＳＩＬ－２Ｒ水平上升的快．但恶性肿瘤患者与正常人ＰＢＭＣ在各种条件激活培养的上清中ＳＩＬ－２Ｒ水平无显著差异．

Serial determinations of soluble interleukin-2 receptor(SIL-2R)levels in sera and supernatants ofperipheral blood mononuclear cell ( PBMC )cultures with PHA or rlL-2 or without any of the stimulantsadded were carried out.The results showed that SIL-2R levels in sera of malignant tumor patients were sig-nificantly higher than those of normal donors.SIL-2R levels in the supernatants of PBMC cultures withoutstimulants from both malignant tumor patients and normal donors were slightly elevated, with the latter rela-tively higher than the former.SIL-2R levels in the supernatants of PBMC cultures with PHA or rIL-2 weremarkedly increased,with the former being increased more rapidly t han the latter while no significant differ-ence was observed between that from malignant tumor patients and normal donors.