期刊文献+

茶叶及强化锌对运动大鼠心肌线粒体H~+转运ATP酶活性及膜流动性的影响 被引量:4

Effects of tea and fortified zinc on the activity of H+-transporting ATPase and membrane fluidity in myocardial mitochondria in exer-clse rats
下载PDF
导出
摘要 为进一步研究锌在运动训练中的作用及机理,作者将30只雄性SD大鼠,随机分为饮自来水对照(A)组、饮茶水(B)组和饮茶水强化锌(C)组.15d后在鼠跑台上以27m/min,8°角跑45min后立即宰杀,取出心脏,分离亚线粒体,测定膜的荧光偏振度,H+转运ATP酶活性及寡霉素的抑制率.结果表明,B组大鼠荧光偏振度、H+转运ATP酶活性及寡霉素的抑制率较A组均有所升高,C组大鼠三项指标均明显高于对照组[0.27±0.01vs0.26±0.01,P<0.05;8.31±0.94vs6.62±0.89μmol/(min·mgprotein),P<0.05和86.55±2.59vs76.54±3.54%,P<0.01].结果提示,茶水强化锌可以增强心肌线粒体膜的稳定性,提高心肌线粒体的能量转换功能. In the present paper,an investigation was carried out to further study the function and mechanism of zinc.Thirty male SD rats were randomized into 3 groups. Rats from Group A,B and C drank tap water,/ayang tea and Ziyang tea fortified with zinc(50 mg/L),respectively, and, 15 d later,rats were slaughtered anmediately after they had run on the treadmill at the speed of 27 m/min and at the angle of 8 for 45 min for detection of membrane fluidity of submitochondria in myocardiocytes,the activity of H+-transporting ATPase and its sensitivity to oligomycin.The findings revealed that the activity of H+-transporting ATPase and oligomycin inhibition rate and fluorescence polarization(P)in Group B after exercise were slightly higher than that of the Group A,and the aforementioned parameters in Group C were significantly greater than those of the Group A[8.31±0.94 vs 6.62±0.89 μmol/(min·mg protein),P<0.05;86.55±2.59 vs 76.54±3.54%,P<0.01 and 0.27±0.01 vs 0.26±0.01,P<0.05].The findings demonstrated that tea and fortified zinc might increase membrane stability of mitochondria in myocardiocytes and improve energy transi-tion in mitochondria in myocardiocytes.
出处 《第四军医大学学报》 1994年第3期206-207,共2页 Journal of the Fourth Military Medical University
关键词 茶叶 膜流动性 心肌 线粒体 zinc tea H+transporting ATPase membrane fluidity exercise test
  • 相关文献

参考文献2

二级参考文献1

共引文献7

同被引文献23

  • 1沈生荣,杨贤强,赵保路,忻文娟.茶多酚体外助氧化作用的自由基机理[J].茶叶科学,1992,12(2):145-150. 被引量:36
  • 2李立中,吴基良,闵清,郑敏,刘忠民,何丽娅.茶多酚对大鼠脑缺血再灌注损伤的保护作用[J].中药药理与临床,1996,12(3):18-20. 被引量:10
  • 3彭章平,黄元伟.茶在心血管疾病防治研究的若干进展[J].心血管病学进展,1995,16(6):329-331. 被引量:6
  • 4龚书明,人民军医,1993年,9卷,增刊,14页
  • 5李文选,军事劳动疲劳研究论文汇编,1992年
  • 6冯美云,中国运动医学杂志,1987年,6卷,4期,222页
  • 7Levitsky J,Gurell D,Frishman WH,et al. Sodium ion/hydrogen ion exchange inhibition:A new phamacologic approach to myocardial ischemia and reperfusion injury[J]. J clin pharmacol,1998,38(10):887-897.
  • 8Stoner GD,mukhtar H.Tea polyphenols as cancer chemopreventive agents[J]. J cell Biochem suppl,1995,22:169.
  • 9Dhalla NS,ElmoselhiAB,Hata T,et al. Status of myocardial antioxidants in ischemia reperfusion injury[J]. Cardiovasc Res,2000,47:446-456.
  • 10Blann AD,Taberner DA. A reliable marker of endothelial cell dysfunction: Does it exist[J]? Br J Haematol. 1995,90(2):244-248.

引证文献4

二级引证文献35

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部