人乳头状瘤病毒原位杂交在尖锐湿疣诊断中的应用

Use of the human papilloma virus in situ hybridization method in thediagnosis of condyloma accuminata

作者用地高辛配基标记人乳头状瘤病毒（ＨＰＶ６．１１）探针，原位杂交方法，对１５０例临床诊断尖锐湿疣（ＣＡ）的活检标本进行病毒学检测，并与病理形态学诊断结果进行比较性研究．结果：病理形态诊断符合ＣＡ者５６例（３７．３％），其中ＨＰＶ阳性者４０例（７１．４％），ＨＰＶ阴性１６例（２８．６％）；无典型ＣＡ病理形态改变者９４例（６２．８％），其中亦有１２例ＨＰＶ呈阳性反应（１２．８％），余８２例阴性（８７．２％）．结果提示：ＣＡ患者中，部分病例缺乏特异性临床表现及病理形态改变，原位杂交技术为ＣＡ的重要辅助诊断方法．

A comparative study between the pathological diagnosis and the virus detection was performed inthe samples of 150 cases diagnosed condyloma acuminata(CA),using in situ hybridization method with theprobe labelled by digoxigenin-11-dUTP.In regard to the pathological diagnosis,40 cases of 56 CA and 12cases of 94 pseudocondyloma showed positive of human papilloma virus with in situ hybridization,The resultsindicate that not all patients of CA have typical clinical feature and morphological changes.We conclude thatthe technique of in situ hybridization is an important method in the diagnosis of CA.