一氧化氮合酶在小鼠肠道壁的分布

The histochemical localiztionb of nitric oxide synthase in the mouseintestines

作者应用ＮＡＤＰＨ硫辛酰胺脱氢酶组织化学方法对一氧化氮合酶（ＮＯＳ）在小鼠肠道壁（十二指肠、空肠、回肠和近段结肠）的分布进行了观察。结果：ＮＯＳ阳性反应产物主要分布于肠神经成分内，大量的ＮＯＳ阳性胞体和纤维分布于肠肌丛，密集的ＮＯＳ阳性纤维见于深肌丛中，只有少数ＮＯＳ阳性胞体和纤维散在地分布于粘膜下层。ＮＯＳ阳性反应产物还见于血管内皮、肠腺腺腔内以及肠粘膜上皮表面，提示肠道壁的ＮＯ可能参与肠道的运动和血液循环的调节过程。

In the present study ,the localization of nitric oxide synthase(NOS) in the mouse intestines(duo-denum,jejunum, ileum and proximal segments of large intestine)was observed using NADPH-diaphorase(ND) histochemistry. The results showed that the NOS-positive reaction product was mainly located in theneuronal elements. Densely stained NOS -positive neuronal bodies and fibers were found in the myentericplexus and dense NOS-positive fibers were distributed in the deep muscular plexus. Only a few of NOS-posi-tive neuronal bodies and fibers were seen scattered in the submucosal laminae。 NOS-positive reaction productwas also seen in the endothelium of small arteries, cavity of the intestinal gland and on the mucosal epithel-um, The results suggested that the NO miglit be involved in the modulation of motility and blood circulationof the intestines。