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粘附式细胞仪测试高温对培养巨噬细胞DNA荧光强度的影响 被引量:3

Analysis and test of fluorescence intensity of heat-treated mouse peritoneal cavity macrophages with adherent cell and sorting cytometer
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摘要 将体外培养4-5天的小鼠腹腔巨噬细胞分成4组,分别置于37℃、39℃、41℃、43℃恒温环境再培养2h。细胞用4,6-二脒基-2-苯基吲哚(DAPI)染色后,用ACAS570激光细胞仪进行测试和分析。以综合荧光量和平均荧光量作为判断高温对细胞中DNA荧光强度影响的主要参数,结果显示,39℃组细胞中DNA荧光强度降低,但41℃、43℃组的又缓慢上升,形成弧形曲线。 unming mouse peritoneal cavity macrophages were cultured for 4-5 days in vitro. The cells were then divided into 4 groups,and incubated at 37 ℃,39 ℃, 4l℃and 43℃for 2h respectivly. The fluorescence intensity of the macrophages was analysed and testedwith adherent cell analysis and sorting cytometre after staining with the DNA-specific dye 4,6-diamidino-2-phenylindole(DAPI).The integrated and average fluorescence values were taken as the main parameters of DNA fluorescence intensity changes of heat-treated cells,The results suggested that the fluorescence intensity of 39℃-treated cells was lower as compared with the control(37℃ ),but the fluorescence intensity of 41℃- treated cells rose slowly again,and, therefore,formed a curve with the shape of an inverted saddle.
出处 《第一军医大学学报》 CSCD 1994年第1期46-47,共2页 Journal of First Military Medical University
基金 国家自然科学基金
关键词 激光细胞仪 高温 巨噬细胞 DNA adherent cell analysis and sorting cytometer heat-treated macrophages DNA fluorescence intensity mice
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参考文献2

  • 1徐湘民,基因的分子生物学,1992年
  • 2高天礼,细胞生理学,1984年

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