松鼠猴的体外受精研究

THE FERTILIZATION IN VITRO OF SQUIRREL MONKEY

本研究用松鼠猴进行试验,探索灵长类体外受精胚胎染色体、生物化学和发育生物学诸问题,为实际应用提供科学依据。结果表明:松鼠猴的体外受精率约为54%,30%分裂为2-细胞期,其中52%为3～4-细胞期。染色体的畸形率为7～16%,三倍体的发生率为16.7%。核糖核酸和蛋白质的合成率与实验动物体外受精胚胎相似。受精后雌激素的结合率增加,但统计学上差异不显著。体外受精时增加孕酮吸收量,并一直维持至2-细胞期。松鼠猴体外受精胚胎与其它灵长类(包括人)体内和体外受精胚胎相比较,在发育时间上基本一致,此即证明松鼠猴胚胎发育可作为灵长类早期胚胎发育的模型。

The studies were carried out with adult squirrel monkeys for the examination of the chromosemal, biochemical and developmental rates of the embryos produced by in vitro fertilization.A fertilization rate approximating to 60% was developed in this species with 30% of the embryos proceeding to the two cell stage and 50% of these to the four cell stage. 9% to 16% chromosomal abnormalities were found. An incidence of triploidy of 16.7% was observed. RNA and protein synthetic rates appeared to be comparable to other laboratory species subjected to in vitro fertilization. Similarly, increase in estrogen incorporation appeared after fertilization but statistically not significant. Progesterone uptake increased in in vitro fertilization up to two cell stage.The developmental rates of in vitro fertilized squirrel monkey embryos is fundamentally similar to the embryos in vitro or in vitro fertilized of other primates and human. It demonstrates, therefore, that the development of in vitro squirrel monkey embryo can be used as a model for the early embryo development of primates.