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刀豆素A诱导小鼠腹腔巨噬细胞内吞与细胞内游离钙的关系

The relationship between endocytosis of mouse peritoneal macrophages induced by ConA and intracellular Ca2+ level
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摘要 采用电镜和粘附式细胞仪(ACAS570)观察了胶体金标记的刀豆素A(ConA-Au)与小鼠腹腔巨噬细胞(Mφ)表面ConA受体的结合、内吞及其在Mφ中转运的形态学改变,同时分析ConA刺激后Mφ内游离钙(Ca2+)的变化。ConA孵育1min后,配体进入内吞小泡,第2次内吞约需20min。粘附式细胞仪分析表明:ConA刺激后50s,Ca2+急剧上升,平均住值达0.557U;约100s后,恢复到刺激前水平;18min后,Ca2+重新回升,持续约18min后开始下降。本实验揭示配体再内吞与Ca2+重新上升的时间相吻合,其肯定性和机理有待于进一步研究证实。 In this experiment the morphological changes during the conjugation, endocytosis and transport ofcolloidal gold-lebelled ConAConA-Au ) with the ConA receptors which are located on the surface ofmouse peritoneal macrophages were investigated. Meanwhile , the change of Ca2+ level in the macrophageafter stimulation with ConA was analysed using ACAS570.The morphological observations showed that 1 min after the inculation of macrophages with Con-Au,some of the ligands entered the vesicles , and the second entry was found about 20 min later. ACAS570anaslysis indicated that 50 sec after the stimulation with ConA , the intracellular Ca2+ concentration roserapidly, the average peak value is 0. 557u. About 100 sec later, Ca2+ regained to the normal level; but itrose again after 18 min or so, maintained for 18 min,and then started to descend. The result implied thatthe time for ligand reendocytosis accorded with the time when Ca2+ rose for the second time. More researchis necessary in order to confirm this point。
出处 《第一军医大学学报》 CSCD 1994年第2期99-101,共3页 Journal of First Military Medical University
关键词 刀豆素A 巨噬细胞 细胞内 游离钙 ConA macrophage endocytosis intracellular free Ca++ ACAS570
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参考文献2

  • 1Hu W,Acta Histochem Cytochem,1991年,24卷,435页
  • 2Wang J J,Acta Histochem Cytochem,1989年,22卷,77页
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