电镜标本快速包埋方法

A rapid embedding method for electron microscopic samples

电镜标本常规包埋过程比较复杂、费时，一般需６０ｈ。本实验采用高温快速包埋法。它是根据物质受热后分子运动加速，包埋介质粘稠度降低，缩短了浸透时间，减少了实验步骤。整个包埋过程仅用８ｈ，而超微结构观察与常规方法比较没有明显差别，大大提高了工作效率。

The conventional embedding procedure for electron microscopic samples is complicated and time-con-suming. Usually, it takes 60 hours.A rapid high-temperature embedding method described here is basedon the acceleration of molecular movement in the material and decline of viscosity of embedding medium athigh temperature. The immersion time was shortened and a few steps were omitted in the new method.Only 8 hours did it take to finish the whole embedding procedure, Moreover,the ultrastructure had no ob-vious difference from that of the samples prepared by the conventional method.Therefore,this method cangreatly elevate the working efficiency.