摘要
建立了大白鼠全脑缺血动物模型，用ＲＩＡ法及荧光分光光度计检测了缺血脑组织匀浆的ＬＥＫ、β─ＥＰ、ＤｙｎＡ１─１３及５─ＨＴ和５─ＨＩ─ＡＡ的含量。ＬＥＫ在脑组织缺血６０ｍｉｎ内含量略下降，但无统计学意义；β─ＥＰ在脑组织缺血３０ｍｉｎ时显著下降（Ｐ＜０．０５），缺血６０ｍｉｎ时明显上升，而５─ＨＴ含量则显著下降。上述结果提示内啡肽和单胺类介质均参与了缺血性脑损伤的病理过程。
ats were used to establish a model
of cerebral ischemia and the changes of monoamines(5─HT,5─HI─AA)and endogenous
opiate peptides(LEK,β─EP,DynA1─13)levels in the cerebral tissue were measured. The main
findings were as follows: The level of LEK reduced slightly, but the β─EP and the DynA1─13
levelsincreased significantly in cerebral ischemia for 60 minutes. At the same time the Level of
5─HT was de─creased and of the 5─HIAA was increased. The results suggest that both the
endogenous opiate peptidesand monoamines play a possible role in the development of
cerebral ischemic damage.
出处
《第一军医大学学报》
CAS
CSCD
1994年第4期266-268,共3页
Journal of First Military Medical University
