γ─氨基丁酸─苯二氮卓受体α＿1亚单位基因的分离、克隆及cDNA序列分析

Isolation, cloning and cDNA sequencing of GABA─benzo─diazepine receptor α1 subunit

报道了大鼠大脑γ─氨基丁酸─苯二氮受体α１亚单位基因的分离、克隆，ｃＤＮＡ序列以及相应的氨基酸残基序列。结果表明在４２８个氨基酸残基长度的成熟多肽链中含有４个跨越细胞膜的疏水性肽段以及２个细胞膜外的Ｎ─糖基化位点。大鼠α１亚单位基因与人和牛的相应基因的氨基酸序列的同源性高达９９％。

Cloned cDNA encoding the rat α1 subunit of GABA─benzodiazepine receptor has been isolated. The cD─NA sequence and the deduced amino acid sequence is presented.The predicted 428 amino acid long mature protein contains 4 postulated membrane─spanning hy─drophobic sequences and 2 putative N─linked glycosylation sites. The rat α1 subunit displays 99%sequencyidentity with the corresponding human and bovine receptor subunits.