反义寡核苷酸对乙型肝炎病毒基因表达的影响

Inhibition of hepatitis B virus by antisense oligodeoxynucleotide

将乙肝病毒（ＨＢＶ）的反义或正义寡核苷酸，加入能复制ＨＢＶ的肝癌细胞模型ＨｅｐＧ２．２．１５中，通过检测其上清中ＨＢｓＡｇ和ＨＢｅＡｇ的含量，初步分析了反义寡核苷酸潜在的抗ＨＢＶ能力。针对Ｃ基因区１９７４─１９５５的反义链（ＣＳＰ）在１５μｍｏｌ／Ｌ浓度下可抑制８４．８％的ＨＢｓＡｇ表达和４０．０％的ＨＢｅＡｇ表达，停药２日时抑制率仍分别为９３．５％和３９．０％。实验期间未发现反义链对上述细胞形态或细胞生长状况有明显影响。结果提示反义寡核苷酸ＣＳＰ在实验条件下具有一定的抑制细胞ＨＢＶ表达的能力。

Sense and antisense oligodeoxynucleotides designed according to hepatitis B virus(HBV) DNA weretested for their potential ability of inhibition of HBV gene expression by detecting HBsAg and HBeAg inthe supernant of an HBV transfected cell line HepG2.2.15 which can replicate HBV. The antisense CSP iscomplementary to the nucleotides 1974─1955 of HBV DNA in C region. And it inhibited 84.8%HBsAg and40.0%HBeAg expression at 15μmol/L. Two days after the withdraw of CSP the inhibitions were still 93.5%and 39.0%respectively. There were no or only minor inhibitions found in the two sense oligodeoxynu─cleotides. There were no side effects on the shape or the growth of the host cells at the given concentration. The results suggest host that antisense CSP have some inhibition effects to HBV.