输精管结扎术后睾丸组织学变化及其机制的实验研究

EXPERIMENTAL STUDIES ON THE POSTVASECTOMY HISTOLOGIC CHANGES OF THE TESTIS AND THE POTENTIAL MECHANISMS

28只输精管结扎家免,以20只同龄雄免作对照。分10、13、16、22月组进行观察。光镜观察表明,10月组曲细精管呈弥漫性萎缩,生精过程停止。16、22月组除少数曲细精管极度萎缩被结缔组织包绕外,大多数曲细精管恢复正常生精过程。电镜发现,术后16、22月组生精过程活跃,Sertoli细胞与Leydig细胞无明显异常。血浆睾酮水平10月组明显低于正常,13月后恢复正常。曲细精管平均细胞数、平均截面积与血浆睾酮水平正相关(P<0.01),与血清抗精子抗体滴度无相关性(P>0.05)。极度萎缩的曲细精管及其周围可见免疫复合物沉积,余者为阴性。

Twenty-eight rabbits were bilaterally vasectomized and were divided to 10, 13, 16, and 22 month postvasectomy groups for histologic investigation with age-matched normal male rabbits as controls. The main results are as follows1. Degeneration and diffuse atrophy occurred in seminiferous tubules 10 months after vasectomy. Spermatogenesis recovered at most seminiferous tubules 16 and 22 months after operation, only a few of the seminiferous tubules having some atrophic sites surrounded by fibrous tissues.2. By electron microscopy, it was found that 16 and 22 months after vasectomy Sertoli's cells and Leydig's cells of the seminiferous tubules showed active spermatogenesis with no marked abnormalities. Furthermore the hematotesticular barries were intact.3. 10 months after vasectomy the plasma testosterone level was significantly lower than normal and began to recover at the 13th months. The average cell amount and the cross section area per seminiferous tubule showed significantly positive correlation sma testosterone (P<0.01), and no correlation with the titer of antibodies against sperm antigen.4. There was deposit of immuno-complexes the inside and around the severly atrophic tubules, it was not found elsewhere.