Ag－NOR电镜染色技术

Ag-NOR Staining Method at the Electron Micros-copical Level

Ａｇ－ＮＯＲ电镜染色技术陆珍凤，周晓军，张太和，喻毅强（南京军区总医院电镜室，南京２１０００２）人胃癌组织的核仁组成区嗜银蛋白（Ａｇ－ＮＯＲ）可以应用一个既简单又可重复的新方法￣［１］，在电镜水平下定位。此法是具有一个乙酰化过程的一步Ａｇ－ＮＯＲ染色...

Silver stainable proteins of the Nucleolar Organizer Regions(Ag-NOR proteins ) of human stomach cancertissues have been localized at the electron microscopical level with a new method which combines a simple andreproducible one step Ag- NOR staining method combined with an acetylation procedure. This new method al-lows the fine identification of nucleolar components,particularly those which are stained by silver. The new method proposed here appears to be a useful tool for the simultaneous study of the localization of ribonucleoprotein and Ag-NOR proteins during the cell cycle.