光棘豆单细胞培养再生植株

Plant Regeneration of Oxytropis Leptophylla in Monocell Culture

选择光棘豆胚轴体细胞无性系中再生能力强的愈伤组织，通过继代培养诱导出不同类型的愈伤组织。将其转入液体培养基Ｓ１２（２．４－Ｄ２ｍｇ／Ｌ，ＫＴ０．５ｍｇ／Ｌ，ＺＴ０．２５ｍｇ／Ｌ）振汤培养，经过滤得到单细胞，２１％蔗糖溶液漂洗纯化后，以１～５×１０￣５个／ｍＬ的密度接种到Ｓ１２培养基中进行液体浅层和双层培养，每７ｄ加液一次，形成愈伤组织后转入增殖培养基；经ＮＢ１２培养基诱导分化出芽，ＭＩ培养基（ＭＳ＋ＩＢＡ０．４ｍｇ／Ｌ）诱导生根，再生完整植株。五种类型的愈伤组织分离的单细胞均能再生细胞团，但只有来源于Ｂ类和Ｃ类愈伤组织的单细胞再生愈伤组织可分化出完整再生植株。

he calli with ahigher capacity of redifferentiation selected from somaclonal calliof Oxytropis leptophylla were transfered to fluid medium S 1 2(2 mg/ L 2,4- D,0.5mg/LKT,0.25mg/LZT)for suspension culture.Agreet number of monocells were seperated from the cell- suspension by the filtration and tnen cultureid in liquid medium.After minicalli were formed from the monocells they were trasfered into the differentiation medium. young shoots were regenrated from the monocells. Derived from cellcospension of C type callus of Oxytropis leptophylla. After the roots were induced on medium (1/2MS+0. 4mg/L IBA), the shoots developed into whole plants. The results indicated that the callus types and cell physical states played an important role in the establishment of cell-suspension lines and in the differentiation and regeneration of plants in monocell culture.