泰山赤鳞鱼同工酶的研究

STUDIES ON THE LACTATE DEHYDROGENASE ISOZYMES OF Varicorhinus sp.

采用琼脂糖凝胶电泳或聚丙烯酰胺垂直板凝胶电泳（ＰＡＧＥ）技术研究了泰山赤鳞鱼早期发育阶段（受精后０—１２０ｈ）及成体眼、脑、心、肾、肝、肌６种组织中的ＬＤＨ同工酶分化表达谱式。结果表明：（１）赤鳞鱼在胚胎发育过程中Ｌｄｈ－Ａ基因和－Ｂ基因同时表达，形成５种不同形式的四聚体（Ｂ４、ＡＢ３、Ａ２Ｂ２、Ａ３Ｂ、Ａ４）。与大多数硬骨鱼相比，赤鳞鱼ＬＤＨ同工酶具有独特的早期个体发育谱式：在整个胚胎发育时期，Ａ亚基与Ｂ亚基的活性几乎相等。（２）赤鳞鱼的ＬＤＨ同工酶谱具有明显的组织特异性。Ｌｄｈ－Ｃ基因仅在肝脏组织表达，以向阴极迁移的分子形式（ＬＤＨ－Ｃ４）特异地表达。

By use of polycrylamide gel electrophoresis. The patterns of expression of LDH isozymes were investigated in six differentialed adult tissue (eye,brain, heart, kidney,liver,and muscle) and the early developmental stages (from zero to 120 h after fertilizied) of Vaicorhinus sp.The results show that: ① During the early developmetal stages LDH-A genes and LDH-B genes exhibited at the same time.Five LDH zones were found.In comparison with most other teleosts,the patternes of expression of LDH isozymes of Varicorhinus sp. have its characteristic during the early developmental stages.The gene activities are equal between A and B.②Isozymes genes in adult Varicorhinus sp.apparently exhibit a tissue-specific expression. The LDH-C gene is only present in liver and it migrates to the cathode.These's LDH-C4. The above results provide basic information for the biochemical genetic survey of Varicorhinus sp. populations and artiflcial breeding of Varicorhinus sp.