鲑鱼促性腺激素释放激素(sGnRH)调节鲤鱼脑垂体生长激素分泌的离体研究

IN VITRO STUDIES OF THE EFFECT OF SALMON GnRH ON THE GROWTH HORMONE SECRETION BY THE PITUITARY OF COMMON CARP(Cyprinus carpio L.)

ＳＧｎＲＨ同时刺激离体灌流孵育的鲤鱼脑垂体ＧｔＨ和ＧＨ分泌，ＧｔＨ和ＧＨ对ＳＧｎＲＨ脉冲式刺激的分泌反应的时间进程和剂量依存关系相似、生长抑素显著抑制基础的和ｓＧｎＲＨ刺激的ＧＨ分泌，但对ＧｔＨ分泌没有影响。阿扑吗啡以剂量依存方式显著抑制基础的和ｓＧｎＲＨ促进的ＧｔＨ分泌，并显著刺激ＧＨ分泌，但对ｓＧｎＲＨ促进的ＧＨ分泌无影响。

The effect of sGnRH on GH and GtH secretion by the pituitary of common carp and the influences of SRIF and apomorphine on basal and sGnRH-induced GH and GtH secretion were studied by using in vitro perifusion system for pituitary fragments. Two-minute sGnRH pulse stimulated simultaneously dose-dependent increases in GH and GtH release from perifused pituirary,with the similar temporal course of GH and GtH release responses. The ED_50s Of sGnRH in stimulating GH and GtH release were 2.85±0.70 nmol/L and 6.24±nmol/L respectively. 100nmol/LSRIF significantly inhibited the basal and 10nmol/L sGnRH-stimulated GH release, but didn't have effect on GtH release. Apomorphine (APO) , Significantly inhibited the basal and 10nmol/L sGnRH-induced GtH release in dose-dependent manner,and significantly stimulated basal GH release, but didn't not have effect on sGnRH-induced GH release.