不同刺激对小鼠B细胞表面IgD表达的影响

REGULATION OF SURFACE IgD EXPRESSION ON MURINE B CELLS

本文通过细胞流式计（ＦＡＣＳ）技术，利用单克隆抗体抗－ＩｇＤ［Ｉｇ（５ａ）７．２］研究了膜表面ＩｇＤ（ｓＩｇＤ）在新制备的小鼠Ｂ细胞上的表达。并通过用不同的丝裂原刺激脾脏休止Ｂ细胞，研究了不同刺激对Ｂ细胞ｓＩｇＤ表达的调节，发现脾脏Ｂ细胞形成两个主要细胞群，高浮力密度小Ｂ细胞高度表达ｓＩｇＤ，而低度表达ｓＩｇＤ的细胞主要是低浮力密度大Ｂ细胞。无论用细菌脂多糖（ＬＰＳ）或抗－ＩｇＭ和白细胞介素－６（ＩＬ－６）刺激脾脏休止Ｂ细胞，均可诱导表面ＩｇＤ降低，同时细胞大量增殖，细胞个体增大，这些结果表明，无论体内或体外，Ｂ细胞活化后表面ＩｇＤ的表达降低。

The expression of surface IgD on murine splenic B cells and the regulation of sIgD expression by different stimuli were studied in this paper. The results demonstrated that splenic B cells form two major populations. The high-density small splenic B cells have the phenotype of sIgD(high) and sIgD(low) B cells fall into low-density large population.Activation of resting B cells with LPS and anti-IgM induces differences in the level of IgD.Stimulation of resting B cells with LPS decreases the level of sIgD, but sIgD remains high after anti-IgM stimulation. Additional stimulation of rIL-6 following anti-IgM stimulation can lead to decrease the level of sIgD. Taking those together,we conclude that the expression of sIgD goes down following B cell activation.