离子对高效液相色谱－TPPS_4光度法测定痕量锰锌铜的研究

Ion-pair HPLC-TPPS_4 Spectrophotometric Determination of Trace Amount of Mn(Ⅱ) t Zn(Ⅱ)and Cu(Ⅱ)

本实验用磺化四苯基卟啉作柱前衍生试剂，研究了在Ｈｇ（Ⅱ）催化下，于ｐＨ５．５时，ＴＰＰＳ_４与Ｍｎ（Ⅱ）、Ｚｎ（Ⅱ）、Ｃｕ（Ⅱ）的配位反应，提出了以苄基三乙基氯化铵作离子对试剂，反相离子对高效液相色谱－光度法分离和测定痕量Ｍｎ（Ⅱ）、Ｚｎ（Ⅱ）、Ｃｕ（Ⅱ）的新方法，检测下限（×１０^（－９）ｇ／ｍＬ）：Ｍｎ^（２＋）０．１５，Ｚｎ^（２＋）０．２０，Ｃｕ^（２＋）０．１１。所确立的方法用于茶汤中锰、锌、铜的测定，结果满意。

MnTPPS_4,ZnTPPS_4 and CuTPPS_4 are formed by reaction of TPPS_4 and the metal ions at pH5.5 using Hg￣(2+)as the catalyst.Then they are separated on a ODS column within 10 minutes using a eluent composed of acetonitrile-water(27.5%)solution containing 50 mmol/L benzyltriethylammonium chloride(ion-pair reagent)and 20 mmol/L acetic acid-sodium acetate(pH6.4)and detected at 415 nm. The detection limit(×10(-9)g/mL) for Mn, Zn and Cu are 0.15,0.20 and 0.11 respectively, Trace amount of Mn, Zn, and Cu in tea fushiom has been successfully determined by this method.