单糖的高效毛细管区带电泳定量分析

Determination of Monosaccharides by Capillary Zone Electrophoresis

生物体中常见的9种单糖经α-萘胺衍生化后,用50mmol/L硼砂作为电泳介质,实现了高效毛细管电泳分离,并以β-吲哚乙酸为内标,研究了9种单糖毛细管电泳的定量方法,结果表明浓度在0.2～4.0mmol/L范围内呈良好的线性。6次进样的标准偏差小于±3％

Reducing monosaccharides were converted into α-naphthylamine derivatives and separated as their borate complexes by capillary zone electrophoresis using a capillary tube (25 μm i. d. 55 cm) of fused silica containing 50 mmol/L borax buffer (pH=10. 5) as car rier. The derivatives of 9 monosaccharides were completely separated in circa 20 min. The detector used is an on-column UV monitoring at 206 nm. The determination of each monosaccharide was carried out using β-indolyIacetic acid as the internal standard. A good linear relationship between the ratio of peak height of the monosaccharide derivative and the internal standard and the concentration of the monosaccharide was obtained in the range of 0. 2-0. 4 mmol/L.