胆红素和胆绿素的电化学行为初探

Investigation on the Electrochemical Behaviour of Bilirubin and Biliverdin

本文用电化学方法和光谱方法对胆红素和胆绿素在磷酸钠溶液（ｐＨ１１．５）中的电化学行为进行了研究。发现胆红素在磷酸钠溶液中可在－０．２５Ｖ、－１．３２Ｖ和－１．４７Ｖ（ｖｓ．ＳＣＥ）左右产生三个阴极化波，在－０．２５Ｖ左右产生一阳极化波，其中－０．２５Ｖ左右处的一对波被证实为可逆波，由胆红素与汞（Ⅱ）配合物的氧化还原引起，而另外二波均为不可逆波，由胆红素内吡咯环的氧化还原产生。胆绿素可在－０．３４Ｖ和０．９８Ｖ左右产生极谱波，－０．３４Ｖ处波为胆绿素与汞（Ⅲ）配合物的氧化还原所致，－０．９８Ｖ处波为一催化波。胆红素和胆绿素产生的这些波均具有吸附波特性，可用于它们的分析测试。

In this paper, the electrochemical behaviour of bilirubin and biliverdin has been investigated with electrochemical and spectrophotometric methods. It was foumd that bilirubin can produce three cathodic peaks at -0. 25,-1. 34,-1. 47V(vs. SCE), respectively. The peak (at -0. 25V) was comfirmed to be a reversible wave which resulted from the reduction of Hg(Ⅲ) -bilirubin complex. The peaks at about -1.34V,-1.47V, were ascribed to the reduction of pyrrol rings of bilirubin. All peaks were characteritized with adsorptive wave and can be used for the determination of bilirubin. Biliverdin can produce two peaks at about -0. 34V and -0. 98V. The peak at -0. 98V, was comfirmed to be a catalytic wave.