L—色氨酸—H2O2—ClO^——CTAB流动注射化学发光反应的研究

Study on Chemiliuminescence Reaction of L-tryptopham-H_2O_2-ClO￣--CTAB by Flow Injection Method

本文采用反相流动注射法研究了在阳离子表面活性剂ＣＴＡＢ存在下，Ｌ－色氨酸－Ｈ２Ｏ２－ＣｌＯ－体系的化学发光行为，对影响化学发光强度的诸因素和表面活性剂的增强作用进行了试验和探讨，建立了化学发光测定Ｌ－色氨酸的新方法。线性范围１．０×１０－８～８．０×１０－６ｍｏｌ·Ｌ－１，检测限为４．０×１０－９ｍｏｌ·Ｌ－１（Ｓ／Ｎ＝２）。对合成试样中三种浓度的Ｌ－色氨酸测定的回收率在９７％～１０６％之间，相对标准偏差小于４％。

A new CL reaction of L-tryptophan-H2O2-ClO--CTAB was studied by flow injection method.' The factors of influencing CL intensity and the enhancement of the surfactants were investigated. A new method baesd on the CL reaction for the determination of trace L-tryptophan has been established by optimizing the experimental conditions. The Linear range for the determination of L-tryptophan was 1. 0 × 10-8～8. 0 × 10- 6 mol. L-l, the determination limit was 4. 0× 10-9 mol· L-1 (S/N=2)