薄层扫描法测定增效联磺片中磺胺嘧啶的含量

The Determination of the Content of Sulphadi-azine with Thin-Layer Chrolnatographic Scanner

本文采用岛津ＣＳ－９３０型薄层扫描仪，以紫外反射吸收方式灵敏快速地测定了增效联磺片中磺胺嘧啶的含量。λｓ＝２７０ｎｍ，散射参数Ｓｘ＝２３，薄层厚度０．３ｍｍ；展开剂为氯仿：乙醇∶庚烷（１∶１∶１），平均回收率为９８．０１％，检出限为０．０５μｇ。

A simple and sensiive thin-layer chro-matographic method is described for the determina-tion of sulphadiazine in compound tablets. The con-tent of sulphadiazine was measured at λ=270nmwith a Shimadzu CS-930 TLC scanner in absorptionmode. The TLC plates used were silica gel G(Merck)plates.The thickness was 0.3mm. Chloroform-ethylalcohol-heptane(1∶1∶1) was used for develop-ment.The recovery of sulphadiazine was 98. 01%.The detection limit was 0. 05μg.