四硫富瓦烯为电子媒介体的葡萄糖生物传感器的研究

A Study of Glucose Biosensor Using Tetratbiaful-value as a Mediator

本文采用四硫富瓦烯（ＴＴＦ）作为葡萄糖氧化酶与玻碳电极之间的电子媒介体，把葡萄糖氧化酶因定在Ｎａｆｉｏｎ－ＴＴＦ修饰电极上，制成葡萄糖生物传感器。用这种传感器测定人体血清中葡萄糖的含量，其线性范围在４．０×１０￣（－５）～２．０×１０￣（－３）ｍｏｌ／Ｌ之间，响应时间为２０ｓ。该传感器具有选择性、重现性好，灵敏度高等优点。

An amperometric enzyme electrode for the analy-sis of glucose is described.The biosensor uses te-trathiafulvalene(TTF)as a mediator of electrdntransfer between immobilized glucose oxidase andglassy carbon electrode. The biosensor shows a linearresponse between 4.0×10￣(-5) and 2.0×10￣(-3) mol/Lwith a response time of 20 seconds. The glucosebiosensor has advantages of selectivity and sensitivi-ty。