γ射线所致哺乳动物细胞hprt基因突变分子机理的初步探讨

PRIMARY STUDY ON MOLECULAR MECHANISM OF γ-INDUCED hprt GENE MUTATION IN MAMMALICAN CELLS

为研究电离辐射致突的分子机理，采用限制性片段长度多态性分析法（ＲＦＬＰｓ）观察γ射线诱导的ＣＨＯ－Ｋ１和ＨｅＬａＭＲ突变细胞ｈｐｒｔ基因结构的变化，并用Ｎｏｒｔｈｅｒｎ杂交和狭缝杂交方法分析了ＣＨＯ－Ｋ１突变细胞ｈｐｒｔ基因的表达情况。实验结果提示：电离辐射引起ｈｐｒｔ基因结构损伤并进而导致ｍＲＮＡ转录的缺陷。

By means of RFLPs method, the alterations of hprt gene structure in the mutant CHO-K1 and HeLa MR cells induced by γ-exposure were examined in order to study the molecular mechanism of gene mutation induced by ionizing radiation; expression of hprt gene in CHO-K1 mutant cells were also observed by Northern blot as well as slot blot. This experiment showed that ionizing radiation could result in hprt gene structure change which might lead to deficiency in mRNA transcription.