麻鸭（Anas　platyrhyncha　domestica）输卵管漏斗部分泌物中无机元素含量及酶活力分析

A Study on the Chemical Components in the Oviducal Infundibulum Fluid of Domestic Duck (Anas platyrhyncha domestica)

以产卵期麻鸭为材料，运用结扎输卵管的方法获得漏斗部分泌物，并分析了其中葡萄糖和几种无机元素的含量及酶的活力。结果表明，葡萄糖的含量为７７．２４±２８．０９ｍｇ／１００ｍｌ，Ｃｌ－浓度为９０．３４±３．１０ｍｍｏｌ／Ｌ，Ｎａ、Ｋ、Ｍｇ、Ｃａ、Ｆｅ、Ｃｕ等无机元素分别为１４１．２８±５．４ｍｍｏｌ／Ｌ、２０５±０．４３ｍｍｏｌ／Ｌ， ０．７２±０．１５ｍｍｏｌ／Ｌ，１．８４±０．２９ｍｍｏｌ、６０．７１±１３．７８μｍｏｌ／Ｌ和４．２５±１．２６μｍｏｌ／Ｌ。在漏斗部分泌物中酸性磷酸酶和碱性磷酸酶的活力极微，未能检出。与血清相比，漏斗部分泌物中乳酸脱氢酶及α－淀粉酶的活力较低。丙烯酰胺凝胶电泳结果表明，输卵管漏斗部分泌物中的α－淀粉酶同工酶谱与血清中α－淀粉酶同工酶谱略有差异。

This study was undertaken to determine some of the chemical components in the luminal fluid from the infundibulum of the domestic duck oviduct. The infundibulum fluid was obtained by ligating the infundibulum of the oviduct. The result showed that in the infundibulum fluid, the amount of glucose was 77. 24 ± 28. 09mg/100ml; The concentrations of chloride, sodium, potassium, magnesium, calcium, iron and copper were 90. 34± 3. 10m mol/L, 141. 28±5. 14m mol/L, 2. 05±0. 43m mol/L, 0. 72±10. 15m mol/L, 1. 84±0. 29m mol/L, 60. 71 ±13. 78μ mol/L and 4. 25±1. 26μ mol/L respectively. Several emzyme analyses were also conducted. The activities of acid phosphatase and alkaline phosphatase were very low and could not be detected. The concentrations of lactic dehydrogenase and α-amylase were lower than those in serum. There was a little difference between the polyacrylamide gel electrophoretic α-anylase pattern of infundibulum fluid and that of serum.