摘要
研究了对苯二酚（ＱＨ２）抑制脲酶催化尿素水解反应及其动力学条件．并以尿素与对－二甲基氨基苯甲醛显色反应为指示反应，建立了动力学光度法测定脲酶催化抑制剂ＱＨ２的新方法．其灵敏度为４．０×１０－１０μｇ／ｍＬ，测定范围为０－０．１２μｇ／ｍＬ，操作简便、快速．该法用于测定乙醇溶液中微量的ＱＨ２，获得满意结果．
Trace urease-inhibitor hydroquinone (QH2) can inhibit urea enzymatic hydrolytic reaction, and its kinetic condition is proposed. A new method for the direct determination of trace hydroquinone is obtained by urea and P-dimethylaminobenzaldehyde devoloping action spectrophotomety. The sensitivity of the method is 4.0×10-10μg/mL, the range of determination is 0-0.12 μg/mL for hydroquinone. The method was simple and rapid,and was applied to the determination of trace hydroquinone in high purity alcohol with satisfactory results.
出处
《福建农业大学学报》
CSCD
1994年第4期495-497,共3页
Journal of Fujian Agricultural University
关键词
动力学
光度法
酶催化反应
脲酶抑制剂
对苯二酚
kinetic spectrophotometry
enzymatic reaction
urease-inhibitor
hydroquinone
