脱除柑桔碎叶病病毒的初步研究

Preliminary Study on the Elimination of Citrus Tatter Leaf Virus

对感染碎叶病毒的柑桔植株分别进行茎尖嫁接，热处理和预热处理结合茎尖嫁接的脱毒试验。白天４０℃１６ｈ，夜间３０℃８ｈ，预热处理３０天以上的柑桔植株再进行茎尖嫁接，均能获得良好的脱毒效果。但是，若茎尖取０．４ｍｍ或更长就没有得到脱毒植株。单用茎尖嫁接方法不能脱除碎叶病病毒；单用热处理方法虽也获得过无碎叶病的植株，但要取得满意的脱除效果是很困难的。

Citrus plants infected with tatter leaf virus (CTLV) were treated by shoot-tip grafting (STG), heat treatment, and a combination of STG with pre-heating treatment respectively. CTLV-free plants were successfully obtained from the combination of STG and pre-heating treatment at 40℃ 16h (day) /30℃ 8h (night) for more than 30 days. However, we failed to obtain CTLV-free plants by using a shoot-tip of 0. 4mm or longer as grafting materials. The CTLV-eliminated rate was very low by using only the heat treatment, and CTLV can not be eliminated by STG without pre-heating treatment.