鸡血浆蛋白多态的定量研究

Quantitative Analysis on the Blood Protein Polymonphisms in Chicken

本试验对３个亲本［澳洲黑鸡（Ｂ）、北京白鸡（Ｗ），北京油鸡（Ｏ）、２个单交组［（北京白鸡♂×北京油鸡♀）、（澳洲黑鸡♂×北京油鸡♀）］和１个混合输精组［（北京白鸡＋澳洲黑鸡）♂×北京油鸡♀］的后代，用聚丙烯酰胺凝胶电泳分离其血浆蛋白谱带，并对运铁蛋白（Ｔｆ）和白蛋白（Ａ１ｂ）电泳区带进行扫描定量，在计算机上进行方差分析、相关分析和回归分析。结果表明：亲本的血浆运铁蛋白（Ｔｆ）和清蛋白（Ａ１ｂ）相对百分含量都高于杂交后代，差异显著；混合输精组后代的血浆Ｔｆ％和Ａ１ｂ％分别处于两个单交组后代的Ｔｆ％和Ａ１ｂ％之间；初生重（ＰＷ）、蛋重（ＥＷ）、Ｔｆ％、Ａ１ｂ％相关分析表明，ＰＷ与ＥＷ为正相关０．７２１４，其它的均为负相关；初主重（ＰＷ）为因变量，ＥＷ、Ｔｆ％、Ａ１ｂ％为直变量。其回归方程为：ＰＷ＝－０．１４０５（Ｔｆ％）－０．０８４１（Ａ１ｂ％）＋０．６６０６ＥＷ＋４．２８９２。

Four chicken parameters(primary weigth(PW), egg weigth(EW), percentage of transferrin(Tf% ) and per,centage of albumen (A1b%),from scanning electrophoesis atlas of chicken blood protein) haye been studied by variance, corruelation, rearssion and progressive regrssion analysis. The results show that the Tf% and A1b% in the. parents' blood protein are signific antly higher than .the offsprings. The Tf% and, A1b% in group of the offspring fern.lized by mixed semen were between the two simple hybridized groups. The correlation coefficient of PW and EW .was 0.7214, and the other were negatilve correlation The regression and the progressiyely regression equations are PW=-0.1405(Tf%)-0.0841,(A1b%)+0.6606 (kW)+4.892 and PW=-0.0798 (A1b% )+,0.6734 (EW)+2.6015, respectiyely.