葡萄糖异构酶的固定化及其稳定性的研究

STUDIES ON THE IMMOBILIZATION AND STABILITY OF GLUCOSE ISOMERASE

以Ａｌ＿２Ｏ＿３为载体，在底物、Ｃｏ￣（２＋）和Ｍｇ￣（２＋）、明胶的保护下，采用先吸咐后交联的方法固定化葡萄糖异构酶，制得的固定化葡萄糖异构酶静态酶活１００００μ／ｇ以上，稳定性高于只用吸咐法制得的固定化葡萄糖异构酶。

Glucose isomerase can be sorbed on alumina followed by crosslinking with glutaraldehyde; insome cases superior results are obtained by the addition of glucose,metal ions Co￣(2+),Mg￣(2+) andgelatin,The activity of immobilized glucose isomerase is 10200μ/g.The stability of the immobi-lized glucose isomerase is higher than that obtained by the only adsorption method only.