茄病镰刀菌毒素的研究Ⅰ．毒素的分离、鉴定及生物测定

Studies on the Toxins of Fusarium solani I.Isolation,Assay and Bioassay

将从豌豆根腐病病株分离获得的茄病镰刀菌（Ｆｕ）Ｄ－０１６菌株接种在ＰＳＤ培养液中，且２５℃恒温箱内黑暗静止培养１４天后，可获得具有毒性的培养滤液。培养滤液及提取的粗毒素经生物测定都具有植物毒性效应。粗毒素经分离纯化后进行的初步鉴定表明，该菌可产生茄病镰刀菌烯醇和Ｔ＿２、二醋酸草镰刀菌烯醇、Ｈ－Ｔ＿２等毒素。

The Fusarium solani(Mart.)Sacc.strain D-016,which was isolated from the rotdisease of pea,was inoculated in PSC liquid medium and cultured under 25℃ in dark in a incubator.After 14 days stationary culture,the filtrate of culture medium with toxicity could be obtained.Thebioassay showed that both of the filtrate and crude toxin extracted from it had phytotoxicity.The primary assay of the rough toxin illustrated that this fungus could produce the toxins including Neo-solaniol,T2,H-T2 and Diacetoxyscirpenol etc.