鸭胚肝细胞培养的研究

Studies on Duck Embryo Liver Cell Culture

取１５日龄左右的鸭胚肝脏，经清洗、剪碎后，加入最终浓度为０．１２５％的胰酶，３７℃消化１５～２０分钟，以含犊牛血清的１９９培养基为营养液，置含５％ＣＯ＿２培养箱内培养７２小时，可制成鸭胚肝细胞单层培养物，若向培养基中加入１０％的鸭胚尿囊液，能促进细胞的生长，接种鸭肝炎病毒后，感染细胞可在４８小时出现ＣＰＥ，９６小时形成空斑。

This article studied the method and condition of duck embryo liver cell culture.Theliver was taken from a duck embryo about 15 days old.It was washed up and cut into pieces,addedtrypsin to 0.125%final concentration and incubated at 37℃ for 15～20 minutes.Then it was cultured in a nutrient medium based on 199 and containing fetal bovine serum.After it had been incubated in a 5%carbon dioxide incubator for 72 hours,the liver cells grew into a monolayer.If 10% duckembryo allantoic fluid was added to the cultured medium,it could help the cells grow. After being inoculated with duck hepatitis virus,CPE(cytopathic effects)and plagues could be observed within 48 and96 hours respectively.