肿瘤抑制基因P_（53）突变与原发性胆囊癌的关系

A preliminary research on the relationship between mutation of tumor supressor gene P_(53)and development of primary gallbladder carcinoma

为了探索肿瘤抑制基因Ｐ５３突变与原发性胆囊癌发生的关系．本组采用聚合酶链反应一限制性片段长度多态性技术．对９例石蜡包埋的原发性胆囊癌组织进行Ｐ５３基因第７外显子２４９～２５０位密码子ＨａｅⅢ酶切位点有无突变的检测．１例慢性胆囊炎组织作为对照。结果无１例发生该酶切位点的突变。原发性胆囊癌的发生与Ｐ５３点突变的关系尚待进一步研究。

For preliminary reserach onthe relationship between P53 gene mutation andcarcinogenesis and evolution of primary gallbladder carcinoma,we used newly-developedPCR-RFLP technique analysis in 9 cases withprimary gallbladder carcinomas and one chroniccholecystitis for control.The point mutation ofP53 exon 7 was detected by PCR and restrictionexzyme digestion with Hae Ⅲ.No one showedpoint mutation on coden 249 and 250.A multiple step process may exist and multiple genesmay be involved in carcinogenesis of primarygallbladder carcinoma.