磺化竹红菌素对蛋白质荧光猝灭机理的研究

STUDIES OF QUENCHING OF PROTEIN FLUORESCENCE BY SULPHATED HYPOCRELLIN

本文详细研究了磺化竹红菌素对多种蛋白质在溶液状态下的荧光猝灭过程。结果表明，磺化竹红菌素对多种蛋白质荧光猝灭服从Ｓｔｅｒｎ－Ｖｏｌｍｅｒ曲线，实验观察了温度、粘度、ｐＨ值和盐酸胍对荧光猝灭过程的影响。由于磺化竹红菌素是一两性分子，对于不同蛋白具有不同猝灭过程；磺化竹红菌素对蛋白质的荧光猝灭常数Ｋ＿ｑ在１０￣（１３）ｍｏｌ／Ｌ·ｓ￣（－１）左右，这说明，磺化竹红菌素是一种比其它蛋白质荧光猝灭剂更加有效的荧光猝灭剂。

This papre studies quenching of proteins fluorescence by sulphated Hypocrellin(HA-SO_3).The experimental results show that the quenching of protein fluorescence by sul-phated Hypocrellin follows the classical Stern-Volmer equation. The variations of otherconditions such as temperatures,viscosities,pH,guanidine-HCl ionic strength were alsoinvestigated.The sulphated Hypocrellin is an amphibious molecule with hydrophobic and hydrophilic.The quenching of various proteins may be at different mechanism;The quenchfluorescence of protein tryphanyl residues is relatively large rate constant(the K_q valuesrang from 1-2 × 10￣(13)mol/L·s￣(-1)).All results also show that HA-SO_3 is a more efficientquencher than others.