一种新型糖脂单分子膜与伴刀豆蛋白A的分子识别

Molecular Recognition of a New Kind of Glycolipid with Concanavallin A in Monolayer Membrane

一种新型糖脂单分子膜与伴刀豆蛋白Ａ的分子识别张希，沈家骢ＲｕｍｐＥ．，ＲｉｎｇｓｄｏｒｆＨ．（吉林大学化学系，长春，１３００２３）（德国Ｍａｉｎｚ大学有机化学研究所）关键词糖脂，单分子膜，分子识别糖与蛋白质、核酸相比，直到六十年代还只当作贮能和结构的...

A new kind of glycolipid bearing p-aminophenyl-α-D-mannopyranoside was synthesized and the direct visual observation of sulforhodamine-labeled concanavallin A bound to the glycolipid monolayer was achieved by using a speciaily designed miniaturized Langmuir trough On the stage of fluorescence microscopy. The glycolipid was spread at a gas phase,and concanavallin A was injected into the subphase. After incubation for 20 min in 20℃,niany large regular concanavallin A domains(μm level) were observed for the first time. No regular protein domain was found when the dead concanavallin A injected,this indicates that the specific interaction between glycolipid and the protein is responsible for the formation of protein domains.