摘要
一种新型糖脂单分子膜与伴刀豆蛋白A的分子识别张希,沈家骢RumpE.,RingsdorfH.(吉林大学化学系,长春,130023)(德国Mainz大学有机化学研究所)关键词糖脂,单分子膜,分子识别糖与蛋白质、核酸相比,直到六十年代还只当作贮能和结构的...
A new kind of glycolipid bearing p-aminophenyl-α-D-mannopyranoside was synthesized and the direct visual observation of sulforhodamine-labeled concanavallin A bound to the glycolipid monolayer was achieved by using a speciaily designed miniaturized Langmuir trough On the stage of fluorescence microscopy. The glycolipid was spread at a gas phase,and concanavallin A was injected into the subphase. After incubation for 20 min in 20℃,niany large regular concanavallin A domains(μm level) were observed for the first time. No regular protein domain was found when the dead concanavallin A injected,this indicates that the specific interaction between glycolipid and the protein is responsible for the formation of protein domains.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1994年第5期783-784,共2页
Chemical Journal of Chinese Universities
基金
国家自然科学基金委员会青年基金
关键词
糖脂
单分子膜
识别
伴刀豆蛋白
Glycolipld, Monolayer membrane, Molecular recognition