开管柱固定化葡萄糖氧化酶反应器的制备及其在流动注射分析中的应用

Preparation of Open Tubular Immobilized Glucose Oxidase Reactor and Its Application in Flow Injection Analysis

本文将葡萄糖氧化酶化学键合到粗糙化的玻璃毛细管内壁上，用开管柱固定化酶反应器－安培检测器进行葡萄糖的流动注射分析。讨论了流速对流动注射分析体系峰电流和分散度的影响，提出了测量开管柱固定化酶表观活力的方法。

Glucose oxidase was chemically bonded on the inner surface of glass capillary.The glucose was determined in the system of open tubular immobilized enzyme reactor-amperometric detector by flow injection analysis. The effect of flow rate on peak current and dispersion in FIA system was discussed. The method of measuring the apparent activity of the open tubular immobilized enzyme reactor was suggested.