生理液中血卟啉溶液状态的光谱研究

Studies on the State of Hematoporphyrin(HP) in Physiological Condition Solution

本文通过对血卟啉在生理条件下（ｐＨ＝７．２，Ｉ＝０．５ｍｏｌ／ＬＮａＣｌ）水溶液中的光谱变化研究，探讨了其在溶液中的聚集状态。应用Ｋａｓｈａ理论推算出血卟啉二聚体的面－面间距离为０．５２８ｎｍ，面间夹角为２０°，平衡常数为２．８５×１０^（－６）。研究了在光作用下，血卟啉－人血清白蛋白溶液体系的光谱特性，讨论了光谱特性与血卟啉在癌瘤光化学治疗机制间的关系。

The polymeric state of hematoporphyrin (HP) under physiological conditions and in aqueous solution has been studied by spectrum analysis. From application of Kasha's theory on the difference spectrum between HP monomer and dimer, we have estimated that the distance between xanthene planes of HP is 0.528 nm and these planes form the cross angle 20°in dimer molecules (Fig. 3). The relationship between the energy transmission of HPHSA and the solution state is discussed. The relationship between the photochemical mechanism of curing malignant tumours and the fluorescence character of HP-HSA is discussed.