激光回射束干涉相移效应的研究

The Study of Retroreflective Laser Beams Interference Phase-Shift Effect

激光回射束干涉相移效应的研究邓延倬，何金兰（武汉大学分析测试科学系，武汉，４３００７２）关键词激光，折射指数，干涉相移效应，毛细管电泳基于激光回射束干涉相移效应，曾首次用于光热测量［１］和微体积样品检测［２］．本文讨论这种效应的基本原理及其用于分析化...

This paper describes a novel optical interference effect, namely retroreflective laser beams interference phase-shift effect. When a focused He-Ne laser beam passes through a cuvette containing liquid sample, the two reflective beams (retroreflective beams)coming from the front and the back surfaces of the cuvette will recombine and generate optical interference effect. The optical-phase-shift between retroreflective beams at the time t depends upon the changes in refractive index of the sample △n. It has been deduced that the relative change of signal intensity at the detector is proportional to △n under the condition of where l is the length of the sample within the cuvette and λ is the laser wavelength.The possibility of determining the small changes in the concentration of micro-volume sample was verified experimentally. Its application to the capillary electrophoresis detector was also demonstrated.