P_（350）微孔快速色谱柱分离富集铀的性能研究

Studies on Properties of Tiny Decompressed Column Chromatography in Separation and Enrichment of Trace Uranium

以铀为例研究了Ｐ３５０萃淋树脂微孔色谱柱的性能．结果表明，柱径和树脂粒度是影响柱效的主要因素，当柱径为２～３ｍｍ，树脂粒度为１２０～１４０目时，上柱流速可为１～８ｍＬ／ｍｉｎ，洗脱体积仅为１ｍＬ．理论塔板高度比内径为７ｍｍ的常现柱下降２个数量级以上，并可节省大量试剂．提出抽空洗脱方式，解决了空床体积影响柱效的难题。

Popular column chromatography suffers some disadvantages such as easy-leaking,great elution volumn and time-consuming.The properties of a tiny decompressed column with P350 levextrel resin in separation and enrichment of uranium are investigated for the first time.The column void volumn is minimized by pumpdown before each elution in order to reach high column performance.The results show that the height equivalent to a theoretical plate(HETP)of the tiny decompressed column(3mm)is reduced 149 times in comparison with popular one, and the elution volume is only 1 mL.