用银和铅标记人白蛋白的离子选择电极电位法研究

A Study of Human Albumin Labelled by Silver and Lead with Ion-Selective Electrodes

用离子选择电极电位法研究了银和铅与人白蛋白（ＨＳＡ）的配合平衡。明确证实银和铅都与ＨＳＡ形成了单核配合物ＭＡ和ＭＡ２．Ａｇ－ＨＳＡ配合物的稳定性积β（１１）＝４．４×１０７，β（１２）＝１．０×１０（１２）；Ｐｂ－ＨＳＡ配合物的β（１１）＝３．１×１０５、β（１２）＝２．０×１０９。根据ＨＳＡ与人白蛋白羊抗血清（ＧＡＨＡ）的免疫反应，用银和铅分别标记ＨＳＡ时，测定ＧＡＨＡ（效价１：４０）的线性范围是０．２～２．０μＬ和０．４～２．８μＬ；而用银和铅分别标记ＧＡＨＡ时，测定ＨＳＡ的线性范围是０～２．９×１０（－８）ｍｏｌ／Ｌ和０～７．３×１０（－７）ｍｏｌ／Ｌ。

The complex equilibria of Ag-human albumin (HSA) and Pb-HSA were studied by ion-selective electrodes.It was clearly proved that both Ag and Pb with HSA formed mononuclear complexes MA and MA2 respectively.The stability products of Ag-HSA complexes are β(11)=4.4×107 and β(12)=1.0×10(12);and that of Pb-HSA complexes are β(11)=3.1×105,β(12)=2.0×109.According to the immunochemical reaction of HSA with albumin antiserum (GAHA),the linear range of determing GAHA(titer 1 : 40)is respectively 0.2-2.0μL and 0.4-2.8μL when HSA is labelled by Ag and Pb; moreover the linear range of determining HSA is respectively 0-2. 9×10(-8)mol/L and 0-7.3×10(-7)mol/L when GAHA is labelled by Ag and Pb.