分离蛋白质的高效离子交换色谱填料的研究（Ⅱ）──新型弱阳离子交换色谱填料的合成及性能

Studies on the New Packings of High Performance Ion Exchange Chromatography for the Separation of Proteins(Ⅱ)──The Synthesis and Characteristics of the New Packings or Weak Cation Exchange Chromatography

将硅胶与环氧基有机硅烷反应的产物与聚乙烯亚胺交联，再与环状酸酐反应制得高效弱阳离子交换色谱填料ＷＣＸ－５和ＷＣＸ－６；交联产物与聚琥珀酰亚胺反应，然后碱性水解制得填料ＷＣＸ－７。研究了它们的性能，ＷＣＸ－５的分离性能优于ＷＣＸ－６，ＷＣＸ－７有一些不同于前两者的色谱特性，三者皆可用于蛋白质的分离，溶菌酶的活性回收率大于９４％。

The studies on the synthesis and chromatographic characteristics of three kinds of new packings of high performance weak cation exchange chromatography for the separation of proteins have been reported on the basis of previous work.The silica reacted with γ-glycidoxypropyltrimethoxy silane,then the product reacted with polyethyleneimine and yielded a crosslinked product,which reacted with a cycloanhydride to give WCX-5 and WCX-6 packings;If the crosslinked product reacted with polysuccinimide,followed by hydrolysis,the WCX-7 packings have been prepared.The crosslinkage of packings ligand could raise the recovery of the bioactivity of proteins,The recovery of the bioactivity of lysozyme is over ninety four percents.The column capacity of WCX-7 packings is bigger than that of other two packings because the crosslinked ligand of WCX-7 is more than that of other two. The reten tion rule of some proteins on the WCX-7 column is different from that of the ordinary WCX column.The retention of lysozyme and Cytochrome-c increases with the increase of pH value,The separation property of WCX-5 is the best.