人X染色体2bA3－YAC克隆重叠群的构建及其与DMD－YAC的串联

AYAC Contig Surrounding 2bA3 on Human X Chromosome Linking to DMD YAC Conting

用Ａｌｕ－ＰＣＲ指纹图谱法分析了人Ｘｐ２１．１一２１．２上一系列的ＹＡＣ克隆，发现其中的两个ＹＡＣ克隆构成包含ＤＸＳ１６６位点的重叠群，而且这一重叠群与以前构建的包含ＤＭＤ基因全顺序的ＹＡＣ重叠群相连接，ＹＡＣ克隆未端探针杂交证实了这一重叠，使这一ＹＡＣ重叠群至少延伸至ＤＸＳ１６６位点，形成一个跨度为３．５Ｍｂ的ＹＡＣ大重叠群。这一工作为这一区域进一步的图谱分析及ＤＭＤ基因的５′远端调控和该区域未知基因克隆奠定了基础。

AYAC contig on human Xp2 1.1一21.2 surrounding the DXS I66 site are analyzed using twostepAl uPCR fingerprinting method,which link to DMD YAC contig constructed before.The overlappingresuIts are also confirmed by YAC end probe cross hybridization and the DMDYAC contig are extended atleast to DXS 1 6 6 site covering 3. 5 Mb. This work facilitates further mapping analysis and clon ing longupstream transtrictional elements of DMD gene and unk nown genes in this region;