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酶化学法制备AraA 被引量：5

Preparation of AraA by Enzyme-Catalyzed Reaction
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摘要 AraA中文名称为9-β-D-阿拉伯呋喃糖基腺嘌呤，通常简称为阿糖腺苷，是一种广谱嘌呤核苷类抗病毒药，具有抑制病毒和肿瘤细胞生长的能力。目前，我国主要采用化学全盛法生产AraA。然而用化学合成法生产AraA反就步骤多。 Two compounds, 1-β-D-arabinofuranosyl uracil (AraU) and 9-β-D-arabinofu-ranosyl adenine (AraA, an antiviral drug) were prepared. AraU was prepared from uridine. AraA was synthesized from AraU and adenine by Upase(uridine phosphorylase, EC 2,4,2,3) and PNPase (purine nucleoside phosphorylase, EC 2,4,2,1). As a possible source of enzyme, Escherichia coli. B23 was found to be able to transfer the arabinofuranosyl moiety of AraU to adenine.
作者 冯万祥 周兴明
机构地区 华东理工大学生化工程系
出处 《工业微生物》 CAS CSCD 北大核心 1994年第4期11-14,共4页 Industrial Microbiology
关键词 阿糖腺苷 抗病毒药 酶化学法 AraA, AraU, Biological catalyst
分类号 TQ464.6 [化学工程—制药化工]
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参考文献1

  • 1Kenzo Yokozeki,Shigeru Yamanaka,Takashi Utagawa,Koichi Takinami,Yoshio Hirose,Atsuo Tanaka,Kenji Sonomoto,Saburo Fukui. Production of adenine arabinoside by gel-entrapped cells of Enterobacter aerogenes in water-organic cosolvent system[J] 1982,European Journal of Applied Microbiology and Biotechnology(4):225～231

同被引文献22

  • 1郭勇莉,丁庆豹,欧伶,邱蔚然.酶法合成抗病毒药物阿糖腺苷[J].生物技术,2006,16(1):32-35. 被引量：7
  • 2蔡武城.生物化学实验技术教程[M].上海:复旦大学出版社,1987.119.
  • 3Lee W W, Benitez A, Goodman L, et al. Synthesis of the β - anomer of 9-(D-ambinofuranosyl)- adenine[J] .J, Am. Chem. Soc., 1960,82:2648-2649.
  • 4Utagawa T, Mofisawa H, Yoshinaga F, et al. Microbiological synthesis of adenine arabinoside[J] .Agric. Biol. Chem., 1985,49(4): 1053 - 1058.
  • 5Utagawa T. Enzymatic preparation of nucleoside antibiotics [ J ]. Journal of Molecular Catalysis B: Enzymatic, 1999, 6:215 - 222,.
  • 6Krenitsky T A, Koszalka G W, Thttle J V,et al. An enzymic synthesis of purine D-arabinonucleosides[J].Garbobydrate Research,1981,97:139-146.
  • 7Feng Ling, lnoue Y, Kimura A. Purification and characterization of a novel nucleoside phosphorylase from a Klebsiella. and its use in the enzymatic production of adenine arabinoside[J]. Applied and Enviromental Microbiology,1990,56(12) : 3830 - 3834.
  • 8Giuseppina B, Simona C, Daniela G, et al. Recombinant bacterial strains for the production of natural nucleosides and modified analogues thereof[P]. Italy, PCT Int. WO 00/39307,2000,7,6.
  • 9Rogert M C, Trelles J A, Porro S, et al. Microbial synthesis of antiviral nucleosides using Escherichia coli BL21 as biocatalyst[J], Biocatalysis and Biotransformation, 2002, 20(5) : 347 - 351.
  • 10Vita A, Amiei A, Magni G,Induction of pyrimidine nucleosides metabolizing enzymes in E.coli B[J].Ital.J.Biochem.1983,32(3):145-151.

引证文献5

二级引证文献14

工业微生物
1994年 第4期

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