激光微束技术研究君子兰花粉管中营养核对生殖细胞分裂的作用

Applying the Laser Microbeam Technique to the Study of the Vegetative Nucleus Function on Division of the Generative Cell in the Development of the Male Gametophyte of Clivia nobilis

本文首次采用光束直径为＜１μｍ，波长为３５５ｎｍＮｄ：ＹＡＧ激光微束技术研究君子兰花粉管中营养核对生殖细胞分裂的作用，实验结果表明营养核能与生殖细胞（或精细胞）结合在一起并影响到生殖细胞分裂和精细胞形成（即雄性生殖单位形成）。本技术对研究生殖细胞与营养核的相互关系是非常方便的。

The vegetative nucleus function on division of the generative cell during the development of the male gametophyte of clivia nobilis was first studied by applying the Nd.YAG laser microbeam technique. The experiment revealed that the pollen tube could still grow to a certain length and the generative cell could enter the pollen tube normally after the vegetative nucleus was destroyed by using laser microbeam, but the genetative cell finally stagnated at the prophase or few at the early metaphase of the mitosis. The results directly demonstrate that the vegetative nucleus may have a physical or physiological function on division of the generative cell. This experiment also shows that the laser microbeam technique has a wide practical prospect in research on development of the male gametophyte or more of the plant reproductive biology.