微量注射进样一阶导数火焰原子吸收分光光度法测定耳全血铜含量

DETERMINATION OF COPPER IN WHOLE BLOOD SAMPLES FROM HUMAN'S EAR USING MICROSAMPLE-INJECTION FIRST-ORDER-DERIVATE FLAME AAS TECHNIQUE

本研究应用ＲＣ微分电路对微量注射进样火焰原子吸收分光光度法所产生的吸收信号进行了处理，提高了信号的选择性和方法的灵敏度。初步实验结果表明：将这两项技术结合后，可方便准确地测定人耳全血中低含量的铜元素。与常规法和微量注射进样法相比吸收信号峰高值分别提高２００％和４００％，样品平均回收率为９９．３７％（９４．８～１０３．６％），方法精密度为４．５％。应用该法对人耳全血铜含量进行了测定，其浓度范围与报道值属同一数量级。

Using RC differential electric circuit, this research deals with the signal produced by Microsample injection Flame AAS. The selectivity and the sensitivity of the method were improved.Preliminary result of the research shows that the low content of copper in whole bloodsamples from human' S ear can be detected exactly by combining the two techniques. Comparing this method with Normal FAAS and Microsample injection FAAS, the PH (PeakHeight) of the signal was increased by 200% and 400%, respectively. Average Recovery of 99. 37% (from 94. 80 to 103. 60% ) was achieved. Precision of the method is 4. 5%. Reference value of Cu in ear blood from Chinese students was aquired by the proposed technique based on the limited sample from 25 volonteers with 340. 03±56' 59μl/dL and from 20 volonteers with 322. 04±83. 56μg/dL.