FTIR法研究核糖核酸酶（RNase　A）二级结构随温度的变化

STUDY ON THE SECONDARY STRUCTURE OF RNASE A AT DIFFERENT TEMPERATURES BY FTIR SPECTROSCOPY

本文利用ＦＴＩＲ法研究了牛胰核糖核酸酶（ＲＮａｓｅＡ）二级结构随温度的变化。结果表明，α－螺旋结构随温度的变化较大，β－折叠的结构变化是一个比较复杂的过程。

The secondary structure of RNase A under different temperatures has been studied by FTIR spectroscopy. The broad amide I band was analyzed by using deconvolution and curve-fitting process. Absorption bands were assigned to the present of α-hences, β-sheets,turns, or random structure, contents of the α-helix will decrease gradually with the rising of the temperature (from about 40℃ to 70℃ )' Contents of the sheet will also decrease with the rising of the temperature. Only at the temperature of about 55℃ does the content change take place.