摘要
用蓖麻凝集素(RCA Ⅰ)对3、4、5、6、7、8个月和出生前人胚食管和食管癌组织采用ABC法进行了石蜡切片标记。结果发现,人胚食管上皮在8月前后,有较明显的分化过程。3-7月,RCA Ⅰ所识别的半乳糖残基仅出现在顶部细胞纤毛的膜上,8月起,食管上皮逐渐由纤毛型演变为非纤毛型,RCA Ⅰ染色中见到基底细胞向表面分化出RCA Ⅰ阳性细胞取代RCA Ⅰ阴性细胞。意味着胞膜上半乳糖残基的表现早于上皮细胞形态学的改变。食管癌细胞,RCA Ⅰ受体的分布和着色的深度都与正常细胞不同、染色加深,不仅在胞膜而且在胞浆,都呈现强的阳性反应。结果提示:蓖麻凝集素对食管上皮的发育、分化以及癌变的研究,是有价值的。
The distribution of glycoconjugates in human differentiating esophagus and esophageal carcinoma was investigated by lectin RCA Ⅰ. Terminal galactose, recognized by RCA Ⅰ was only expressed in ciliary membrane of epithelial cells among the third to 7th monthes. The positive cells stained by RCA Ⅰ was first expressed in the 8th month of embryo and gradually replaced the negative stained cells. The development of the saccharidic moieties of glyc(?) jngates precedes the epithelial morphological transformations.The appearance of RCA Ⅰ binding glycoconjugates in cell membrane in human esophageal epithelial cells may represent a metration marker. Terminal galactose was expressed more richly both in cell membrance and in cytoplasm of carcinoma cells. It was suggested that RCA Ⅰ would also be a marker for esophageal carcinoma cells.In conclusion, lectin RCA Ⅰ histochemistry is proved to be useful for the studies of esophageal epithelial cell differentiation and esophageal carcinoma.
