摘要
以核酸杂交法检测了60份脐血标本及46份胎盘组织标本中人巨细胞病毒DNA同源序列,发现脐血阳性14份(阳性率为30.4%),胎盘组织阳性1份(阳性率为2.17%)。用免疫酶染色法测定60份脐血的HCMV抗体(60份标本中46份与脐血和胎盘组织DNA配对),IgG阳性46份(阳性率7l.7%)、IgM阳性者l份(阳性率2.2%),两型抗体均为阳性者l份。本结果证实了HCMV能通过胎盘这种传播途径引起宫内感染的已有结论。
Homologuous human cytomegalovirus(HCMV) DNA sequence was detected in 60 cases of cord blood and 46 cases of plancenta with nucleic acid dot blot hvbridization. 14 samples of cord blood were positive,the positive rate was 2.17%. One sample of placenta was positive,the positive rate was 2.17%. Anti-HCMV antibodies were detected in 60 cord blood samples, 46 among which were from the same donors of the placentae with immuno-enzymatic staining. 46 were positive for IgG, and one was positive for IgM, one was positive for IgG+IgM. The positive rates for IgG ane IgM antibodies were 7l.73% and 2.2% respectively. HCMV exists ubiquito usely in human population. The results confirmed that HCMV could ultilize placenta as a route of transmission to cause intrauterine infection.
出处
《广东医学院学报》
1994年第1期15-16,14,共3页
Journal of Guangdong Medical College
关键词
巨细胞病毒
脐血
胎盘
DNA
杂交
human cytomegalovirus(HCMV)
cord blood
placenta
DNA hybridization
detection of antibodies