人乳头瘤病毒感染的宫颈癌组织的病理形态学观察

The Pathomorphology Study on Cervical Carcinoma Infected by Human Papill omavirus

应用核酸杂交技术结合组织病理学的光镜和电镜观察、检测８０例子宫颈癌、４２例正常宫颈组织、３３例宫颈非典型增生组织和１０例宫颈尖锐湿疣组织中的ＨＰＶｓ基因组相关序列。结果表明：（１）４２例正常宫颈组织ＤＮＡ无一例检出ＨＰＶ，而在宫颈癌组织、宫颈非典型增生组织和宫颈尖锐湿疣组织中，ＨＰＶ检出率分别达４３．７５％、４５．４５％和８０％；（２）宫颈癌组织中阳性３５例，阳性率为４３．７５％；ＨＰＶ型别分布主要为ＨＰＶ１６型（１８例，５１．４３％）和ＨＰＶＲ型（８例，２２．８６％）；（３）１８例ＨＰＶ１６型和３例ＨＰＶ１１／６型均为鳞癌，８例ＨＰＶＲ型中有６例也为鳞癌。ＨＰＶ１６型的１８例中有１２例为低分化癌，ＨＰＶＲ型的８例中有５例也为低分化癌；（４）不同型别ＨＰＶ感染的宫颈癌组织，其病理形态学特征有所不同。研究表明，广西地区相当部分宫颈癌的发生与ＨＰＶ感染有关，其感染的宫颈癌组织具有一定的病理形态学特征。

Eighty biopst specimens of carcinoma of the uterine cervix, fourly-two the normal cervix,thrity-three the atypical hyperplasia and ten the condyloma acuminatum were assayed for HPV DNA related sequences using the southern blot hybridization technique combining observation of light microscopy and electron microscopy, The results showed that:(1) No HPV DNA had been found in the normal uterine cervix; HPV DNA were found to be 43. 75%, 45. 45% and 80% respectively in thecervical carcinoma, the atypical hyperplasia and the condyloma acuminatum; (2) The HPV DNA typeswere mostly HPV 16 (18 cases, 45. 43%) and HPV R (8 cases, 22. 86%); (3) All 18 cases of HPV16 and 3 cases of HPV 11/6 were found to be squamous cell carcinomas. Six of the eight cases withHPV R were also squamous cell carcinoma; (4) The Pathomorphologic features of the cervical cancernous tissues with different HPV types were different. The results also showed that the developmentof quite a few cervical carcinoma in Guangxi might be related to HPV infection.